ISO INTERNATIONAL STANDARD 17780 First edition 2015-08-15 Animal and vegetable fats and oils Determination of aliphatic hydrocarbons in vegetable oils Corp gras d'origines animale et vegétale - Détermination des hydrocarbures aliphatiques en corps gras d'origines vegetale Reference number IS0 17780:2015(E) ISO International Organization for Standardization see=Zhejang Institute of Standardization 5956617 @IS02015 No reproduction or networking permitted without license from IHS IS0 17780:2015(E) COPYRIGHT PROTECTED DOCUMENT IS0 2015, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Ch. de Blandonnet 8 · CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Internatinir DrganizationforStandardization Not for Resale, 2015/11/11 08:22:26 networking permited withoutlicense from IHS IS0 17780:2015(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Terms and definitions 4 Principle ..2 5 Reagents. ..2 6 Apparatus ...3 7 Sampling. .4 8 Preparation of the test sample ..5 9 Procedure .5 9.1 Chromatography column preparation .5 9.1.1 Preparation of AgNO3 impregnated silica gel 5 9.1.2 Column packing, .5 9.2 Elution of the hydrocarbon fraction .5 9.3 Gas chromatography .6 9.3.1 Gas chromatography setup .6 9.3.2 Working conditions for gas chromatography analysis .6 9.3.3 Peakidentification .6 9.3.4 Performance of the gas chromatography system .7 9.4 Procedural blank 9.5 Quantitative determination 10 Determination of hydrocarbons attributed to mineral origin ..11 11 Precision ..11 11.1 Interlaboratory test .11 11.2 Repeatability .11 11.3 Reproducibility 11 12 Test report .11 Annex A (informative) Examples of chromatograms ..12 Annex B (informative) Validation of silver nitrate impregnated silica gel purification. ..17 Annex C (informative) Procedure for rapid method ..19 Annex D (informative) Fat extraction from food sample ..23 Annex E (informative) Results of interlaboratory trials ..26 Bibliography .29 ii icensee=Zhejiang Institute of Standardization 5956617 thout license from IHS