ICS65.202.20 CCSB21DB5301 昆明市地方标准 DB5301/T84—2023 人参果脱毒种苗生产技术规程 2023-04-15发布 2023-05-01实施 昆明市市场监督管理局发布 DB5301/T84—2023 I目  次 前  言............................................................................III 1范围...............................................................................1 2规范性引用文件.....................................................................1 3术语和定义.........................................................................1 4脱毒种苗生产设施、设备及化学试剂...................................................2 4.1组培实验室.....................................................................2 4.2温室...........................................................................2 4.3仪器设备、玻璃器皿及试剂.......................................................2 5常用溶液的配制.....................................................................3 5.1MS培养基母液...................................................................3 5.2生长激素母液...................................................................3 6无菌苗的培养.......................................................................3 6.1无菌苗培养基的制备.............................................................3 6.2田间株选和外植体材料的采集.....................................................3 6.3外植体的处理和消毒.............................................................3 6.4离体培养无菌苗.................................................................4 7茎尖培养脱毒.......................................................................4 7.1钝化病毒.......................................................................4 7.2茎尖培养基的制备...............................................................4 7.3茎尖剥离与接种.................................................................4 7.4茎尖分生组织培养...............................................................4 7.5筛选脱毒核心苗.................................................................4 8基础苗扩繁和保存...................................................................5 8.1基础苗扩繁.....................................................................5 8.2基础苗保存.....................................................................5 9脱毒组培苗快繁.....................................................................5 9.1无菌操作要求...................................................................5 9.2脱毒组培苗接种.................................................................5 9.3脱毒组培苗培养.................................................................6 9.4脱毒组培苗继代或出瓶炼苗.......................................................6 10脱毒组培苗炼苗....................................................................6 10.1炼苗设施......................................................................6 10.2基质配制......................................................................6 10.3炼苗移栽......................................................................6 DB5301/T84—2023 Ⅱ10.4移栽后管理....................................................................7 10.5脱毒组培苗炼苗后质量标准......................................................7 11脱毒种苗扩繁......................................................................7 11.1设施..........................................................................7 11.2基质..........................................................................7 11.3扦插时间......................................................................7 11.4扦插材料来源与剪材要求........................................................8 11.5扦插..........................................................................8 11.6扦插后管理....................................................................8 11.7脱毒扦插种苗..................................................................8 12档案管理..........................................................................8 附录A（资料性）组培室规划........................................................10 附录B（资料性）组培设备、试剂....................................................11 附录C（资料性）MS培养基母液与相关培养基的配制...................................13 附录D（资料性）无菌操作注意事项..................................................16 附录E（资料性）人参果脱毒种苗繁育主要病虫害防治方法..............................17 附录F（资料性）